Oligomerization of a Glucagon-like Peptide 1 Analog: Bridging ...
WEBSep 15, 2015 · The glucagon-like peptide 1 (GLP-1) analog, liraglutide, is a GLP-1 agonist and is used in the treatment of type-2 diabetes mellitus and obesity. From a pharmaceutical perspective, it is important to know the oligomerization state of …
NMR Spectroscopy for Protein Higher Order Structure Similarity
WEBJul 13, 2021 · Here, NMR spectra were collected on marketed peptide and protein DPs, including calcitonin-salmon, liraglutide, teriparatide, exenatide, insulin glargine and rituximab. The 1D 1 H spectral pattern readily revealed protein HOS heterogeneity, exchange and oligomerization in the different formulations.
Direct Assessment of Oligomerization of Chemically Modified …
WEBJan 10, 2023 · DOSY-NMR of Protein DP and Equilibrium Between Oligomers. The diffusion coefficients of all the six chemically modified protein drugs were also measured using DOSY-NMR (Figs. 5 and 6). A DOSY-NMR precision test was performed on liraglutide DP (Table S6) with the resulting CV of 1.1%
The structure and function of the glucagon-like peptide-1 receptor …
WEBA recent NMR analysis of an analogue of Ex4 with enhanced activity (β-Ala replaces Glu-3, while Tyr replaces Gln-3) has shown a longer helical region that extends towards the N-terminus and that may be responsible for its apparent increase in …
Oligomerization, albumin binding and catabolism of …
WEBFeb 20, 2022 · In this work, we have leveraged the use of NMR and SPR spectroscopy to elucidate oligomerization propensity and albumin interaction of different analogs of the two marketed lipidated GLP-1 agonists liraglutide and semaglutide.
Controlled release of liraglutide using thermogelling polymers in ...
WEBAug 17, 2016 · Two triblock copolymers PCGA-PEG-PCGA and PLGA-PEG-PLGA were synthesized by us and characterized via proton nuclear magnetic resonance (1 H NMR) and gel permeation chromatography (GPC).
Liraglutide reduces oxidative stress and improves energy …
WEBSep 1, 2022 · We conducted a nuclear magnetic resonance (NMR)-based analysis of metabolomic profiles and provided insights into the possible mechanisms underlying the effects of liraglutide on diabetes-associated cognitive-impaired brains.
Glucagon-like peptide-1 analog liraglutide leads to multiple …
WEBNov 7, 2022 · A recent NMR spectroscopy–based urine metabolomics study has detected the effects of liraglutide treatment in diet-induced obese (DIO) mice: liraglutide decreased urine levels of nicotinamide metabolites and taurine and increased those of …
WEBChemically modi-fied proteins can have oligomerizations such as the hexa- to octamer formation of liraglutide, [14] monomerization of peginsulin, [10] or oligomerization of pegfilgrastim in growth medium [15].
Coordinated ASBT and EGFR Mechanisms for Optimized Liraglutide ...
WEBJun 29, 2024 · Field-emission scanning electron microscopy (FE-SEM, SUPRA 55VP, Carl Zeiss, Germany) and 2D diffusion-ordered 1H nuclear magnetic resonance spectroscopy (DOSY-NMR) were deployed to validate nanoparticles within the LG/TD-NF.