Western blots have been a tried-and-true method for separating and quantifying proteins based on molecular weight, but they have not kept up with the continually increasing output demands of modern ...

Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak ...

Western blotting (or immunoblotting) is a well-established biochemical technique that detects specific proteins in complex samples and has applications from basic research to the diagnosis of ...

Protein detection and analysis using western blotting is a fundamental and well-established laboratory technique for a wide range of research and clinical applications. Western blotting workflows ...

A capillary western blot (Wes) technology has recently been validated for analyses of cell culture lysate proteins, but whether it is reliable for human tissue proteins is unknown. We compared ...

Western blotting is a foundational technique for detecting specific proteins in a complex sample. Although blotting protocols are straightforward, many critical decision points can influence the ...

A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue or ...

Detection of low-abundance proteins and/or detection of proteins from precious samples can be a major challenge when performing western blotting. The abundance of the target may be low because the ...

Western blotting forms the bedrock of many protein studies, with the technique cited in 9% of all protein related papers. The technique had its 40th birthday in 2019, an event that was widely ...

Reviewed by Michael Greenwood, M.Sc. Fluorescent western blotting is a powerful protein detection technique that offers numerous benefits in comparison to alternative chemiluminescent or chromogenic ...