DNA vs. RNA – 5 Key Differences and Comparison
Jan 22, 2024 · DNA is a much longer polymer than RNA. A chromosome, for example, is a single, long DNA molecule, which would be several centimetres in length when unraveled. RNA molecules are variable in length, but much shorter than long DNA polymers. A large RNA molecule might only be a few thousand base pairs long. Sugar.
UV-Vis Spectroscopy: Principle, Strengths and Limitations and ...
Dec 18, 2023 · Ultraviolet-visible (UV-Vis) spectroscopy is a widely used technique in many areas of science ranging from bacterial culturing, drug identification and nucleic acid purity checks and quantitation, to quality control in the beverage industry and chemical research. This article will describe how UV-Vis spectroscopy works, how to analyze the output data, the technique's strengths and limitations ...
GC-MS Principle, Instrument and Analyses and GC-MS/MS
Feb 16, 2024 · The sample mixture is first separated by the GC before the analyte molecules are eluted into the MS for detection. 1 They are transported by the carrier gas (Figure 1 (1)), which continuously flows through the GC and into the MS, where it is evacuated by the vacuum system (6). 1. The sample is first introduced into the GC manually or by an autosampler (Figure 1 (2)) and enters the carrier gas ...
SEM vs TEM - Technology Networks
Jan 24, 2024 · Even the basic microscope setup is different. In SEM, the sample, located at the base of the electron column, is scanned and the resulting electron scattering is analyzed to produce an image. In TEM, the sample is placed in the middle of the microscope and electrons pass through the sample before being collected.
An Introduction to Culturing Bacteria - Technology Networks
Jan 24, 2024 · Bacteria are an essential part of the ecosystem. They are crucial to our health and the environment, have an important role in food production and provide bioengineers with tools to harness their properties and manufacture compounds. However, they can also be harmful, causing damage and disease. The ability to grow these microbes is therefore ...
LC-MS – What Is LC-MS, LC-MS Analysis and LC-MS/MS
Jul 30, 2024 · Coupling liquid chromatography (LC) with mass spectrometry (MS) has provided scientists with a powerful tool to meet stringent analytical demands and their versatility and efficiency means LC-MS instruments have become desirable in many laboratories. In this article, we consider how LC-MS works, what it tells us and highlight some of the technique's strengths and weaknesses.
Gas Chromatography – How a Gas Chromatography Machine …
Mar 12, 2024 · The final step is the detection of the analyte molecules when they elute from the column. There are many types of GC detectors, for example: those that respond to C-H bonds like the flame ionization detector (FID); those that respond to specific elements for example sulfur, nitrogen or phosphorus; and those that respond to specific properties of the molecule, like the ability to capture an ...
Missense, Nonsense and Frameshift Mutations: A Genetic Guide
Apr 26, 2022 · Missense. Mistake in the DNA code, one of the DNA base pairs is changed. Nonsense. Single change in DNA code produces stop codon, prematurely terminates protein synthesis. Insertion. Addition of one (or more) nucleotide base pairs into the DNA sequence. Deletion. A piece of DNA is removed from the sequence. Frameshift.
Cation vs Anion: Definition, Chart and the Periodic Table
Jan 24, 2024 · Cations are ions that are positively charged. Anions are ions that are negatively charged. Ions are charged atoms or molecules. If a balanced atom loses one or more electrons, it will become a positively charged cation. If a balanced atom gains one or more electrons, it will become a negatively charged anion.
Prokaryotes vs Eukaryotes: What Are the Key Differences?
Jun 26, 2024 · In prokaryotes, DNA is bundled together in the nucleoid region, but it is not stored within a membrane-bound nucleus. The nucleus is only one of many membrane-bound organelles in eukaryotes. Prokaryotes, on the other hand, have no membrane-bound organelles. Another important difference is the DNA structure.